News

May 19, 2016
Category: General
Posted by: antiadmin

Automated assays for biological drugs.

May 19, 2016
Category: General
Posted by: antiadmin
As of May 2016, we are no longer defined as a regional core facility.
 

Purification and labelling of user-supplied antibodies

We can help with:

  • Purification of immunoglobulins by conventional chromatographic methods (ion-exchange, gel-filtration) or affinity techniques (protein-A, biomimetic).
  • Chemical labelling of antibodies with biotin, fluorescent dyes and europium chelates.
  • Fragmentation of antibodies by limited proteolysis (Fab´, (Fab)2) or recombinant techniques (scFv).
  • Radioiodination of antigens and immunoglobulins.

We normally provide these services to investigators on a contract basis.

All antibody reagents supplied will be Quality Controlled by UV absorption and SDS-PAGE. If requested, IEF and high-performance size exclusion chromatography (to assess aggregate content) could also be undertaken.