Purification and labelling of user-supplied antibodies
We can help with:
- Purification of immunoglobulins by conventional chromatographic methods (ion-exchange, gel-filtration) or affinity techniques (protein-A, biomimetic).
- Chemical labelling of antibodies with biotin, fluorescent dyes and europium chelates.
- Fragmentation of antibodies by limited proteolysis (Fab´, (Fab)2) or recombinant techniques (scFv).
- Radioiodination of antigens and immunoglobulins.
We normally provide these services to investigators on a contract basis.
All antibody reagents supplied will be Quality Controlled by UV absorption and SDS-PAGE. If requested, IEF and high-performance size exclusion chromatography (to assess aggregate content) could also be undertaken.