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Mar 31, 2022
Category: General
Posted by: antiadmin
The Department of Medical Biochemistry has updated and moved its assay menu to the new platform ous.labfag.no.
 

Bulk culture and antibody purification

Two approaches are available to produce antibodies:

  • Batch culture of hybridomas in standard T-flask, roller bottles for stirred fermenters. With this method one can expect 10 - 30 mg of purified antibody from a 1 liter (2-3 week) production run.
  • Membrane-partitioned flasks can provide >100 mg of a selected antibody/month.