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Mar 31, 2022
Category: General
Posted by: antiadmin
The Department of Medical Biochemistry has updated and moved its assay menu to the new platform ous.labfag.no.
 

Production of novel monoclonal antibodies

The generation of hybridoma cell lines can be subdivided into five phases:

  • Antigen preparation/immunization
  • Generation of Mab-producing hybridoma cell lines
  • Screening of hybridoma clone supernatants
  • Expansion, cloning and cryopreservation of selected hybridomas
  • Antibody expression and purification from culture supernatants