Hybridomas are generated by PEG-facilitated somatic cell hybridization of splenocytes from the hyper-immunized mice with the NS0 murine myeloma cell line.

The fusion products are distributed into 10 x 96-well microplates in a highly enriched growth medium. We have developed an optimalized RPMI 1640-based medium that permits rapid hybridoma growth while minimizing loss of Mab-producing clones through apoptosis.

After approximately 9 days incubation at 37 oC, the individual culture supernatants are screened for Mabs with the desired antigen reactivity.