The preparation of appropriately hyper-immunized mice is an important first step in Mab production.

Mice will be immunized with the selected antigen. Native proteins, recombinant fusion proteins, peptide conjugates and whole cells can be used for immunization.

In general, ca 1-5 mg of protein immunogen are necessary for immunization. For antigens available in limited amounts (eg some native proteins, FACs-sorted cells), it is our experience that direct intra-splenic immunization with <10 µg total antigen (or 1 x 105 cells) can often generate useful monoclonal antibodies.

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